Random Amplified Polymorphic Dna-PCR Typing of Vibrio parahaemolyticus Isolated from Local Cockles (Anadara granosa)
Lesley Maurice Bilung, Son Radu, Abdul Rani Bahaman, Raha Abdul Rahim, Suhaimi Napis, Cheah Yoke Kqueen, Chandrika Murugaiah, Yousr Abdul Hadi, Tunung Robin and Mitsuaki Nishibuchi
DOI : 10.3844/ajisp.2005.31.36
American Journal of Immunology
Volume 1, 2005
Randomly amplified polymorphic DNA (RAPD) was used in this study to examine the genetic relatedness among the Vibrio parahaemolyticus strains. In the analysis by RAPD-PCR, the size for RAPD fragments ranged from 0.25 to 10.0 kb with average number of ten bands. The RAPD profiles revealed a high level of DNA sequence diversity within the Vibrio parahaemolyticus strains tested. Hence, this study, demonstrated that the local cockles (Anadara granosa) in the study area are populated by genetically polymorphic strains of V. parahaemolyticus. In addition, RAPD-PCR is simple, robust and sensitive typing methods to differentiate the V. parahaemolyticus strains.
© 2005 Lesley Maurice Bilung, Son Radu, Abdul Rani Bahaman, Raha Abdul Rahim, Suhaimi Napis, Cheah Yoke Kqueen, Chandrika Murugaiah, Yousr Abdul Hadi, Tunung Robin and Mitsuaki Nishibuchi. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.