Interleukin 10 Induces the Maturation of Putative Tolerogenic Monocyte-derived Dendritic Cells as Revealed by GeneArray Analysis
Marianne A. Jakobsen, Bjarne K. MØller, Klaus Rieneck, Klaus Bendtzen and SØren T. Lillevang
DOI : 10.3844/ajisp.2005.135.142
American Journal of Immunology
Volume 1, Issue 4
IL-10 is a multifunctional cytokine with known inhibitory effect on DC maturation. In the present study, we show that the presence of IL-10 during the first 6 days of maturation of Mo-DC has severe effects on the phenotype with IL-10 treated Mo-DC retaining a high expression of factors involved in antigen uptake and having low expression of costimulatory factors and MHC II. The effect of IL-10 on DC maturation is further characterized by a comprehensive investigation of the expression profile of 12500 genes using GeneArrays and comparing the data for IL-10 treated and untreated Mo-DC at day 6 and 8 with monocytes and macrophages. The data is confirmed with semi-quantitative RT-PCR. We find that the transcriptional pattern of IL-10 treated Mo-DC at day 6 and day 8 is distinct from both untreated Mo-DC and macrophages. IL-10 induces the expression of genes coding for CCL18, ILT3, ILT4 and TGFβ while inhibiting the expression of IL-12 p40 mRNA, and genes coding for CCL17, CCL19 and CCL22 suggesting a likely effect of IL-10 on Mo-DC maturation to be the generation of tolerogenic Mo-DC.
© 2005 Marianne A. Jakobsen, Bjarne K. MØller, Klaus Rieneck, Klaus Bendtzen and SØren T. Lillevang. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.