Isolation and Mutagenesis of Streptokinase Producing Bacteria
Thaer Taleb Abed Abdelghani, Adinarayana Kunamneni and Poluri Ellaiah
DOI : 10.3844/ajisp.2005.125.129
American Journal of Immunology
Volume 1, Issue 4
Of 103 β-haemolytic Streptococci (with haemolytic activity) in total were isolated from different samples of blood and biomass from infected throat, 47 isolates exhibited streptokinase (SK) activity. T3, identified as a new variant of Streptococcus equisimilis, was found to be excellent SK producer in submerged fermentation and was selected for strain improvement studies. This strain was mutagenized by UV and N-methyl-N'-nitro-N-nitroso guanidine (NTG). The isolation of mutants and the SK activity of selected mutants were described. The SK yield of the best UV mutant AUV10 was 120% higher than the wild strain (T3). Also, the SK yield of the best NTG mutant NUV7 was 146% higher than the wild strain (T3). The results indicated that UV and NTG were effective mutagenic agents for strain improvement of S. equisimilis T3 for enhanced SK productivity.
© 2005 Thaer Taleb Abed Abdelghani, Adinarayana Kunamneni and Poluri Ellaiah. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.