Hemodynamic Catheters: The Reprocessing, Cleanliness and in vitro Biofilm Formation by Enterococcus faecium in a Continuous Flow Model
Cleber Galdino da Silva, Edson Pires de Oliveira, Ednalva Miranda Guizi, Julio Spadotto, Celia Guadalupe Tardeli de Jesus Andrade, Gilselena Kerbauy, Vivian Biazon El Reda Feijó, Elizabeth Silva Ursi, Renata Aparecida Belei, Glenda Cavalari Simões, Alane Tatiana Pereira Moralez, Galdino Andrade and Admilton Gonçalves de Oliveira
DOI : 10.3844/ajidsp.2015.33.40
American Journal of Infectious Diseases
Volume 11, Issue 2
Reprocessing single-use devices to cut costs is a common practice in hospitals around the world. In Brazil, there are few studies of reprocessing hemodynamic catheters and thus, this study aimed to evaluate the effectiveness of reprocessing hemodynamic catheters before and after biofilm formation in vitro using a continuous flow model. We used a sterility test and Scanning Electron Microscopy (SEM) to assess the presence of microorganisms, residue and integrity of a New (NC) and Reprocessed (RC) hemodynamic catheter, before and after in vitro biofilm formation by a clinical isolate of Enterococcus faecium (strain 155). NC was considered the negative control. The sterility test did not show the presence of microorganisms in either catheters used as a negative control (NC and RC). On the other hand, changes in integrity were observed by SEM in the RC, with a large number of microcracks and recesses, indicating that this would get worse after reprocessing. After biofilm formation and subsequent sterilization by ethylene oxide, both catheters were examined by SEM and RC showed a dense array of exopolysaccharide and substantial organic waste material, which was not evident in NC, showing changes in surface integrity. Ethylene oxide sterilization is very efficient in the sterilization process but the reprocessed catheters after biofilm formation by strain 155, showed marked surface changes, which increases the adhesion of organic matter and compromises the cleaning process in reprocessing. The results can be used as a parameter for hospitals and companies that reprocess catheters, to develop protocols for standardized and systematic surveillance in reusing materials recommended for single use to prevent infections.
© 2015 Cleber Galdino da Silva, Edson Pires de Oliveira, Ednalva Miranda Guizi, Julio Spadotto, Celia Guadalupe Tardeli de Jesus Andrade, Gilselena Kerbauy, Vivian Biazon El Reda Feijó, Elizabeth Silva Ursi, Renata Aparecida Belei, Glenda Cavalari Simões, Alane Tatiana Pereira Moralez, Galdino Andrade and Admilton Gonçalves de Oliveira. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.