Anti-Inflammatory Activity of Vitex leucoxylon L. Bark Extracts Against Freund's Complete Adjuvant Induced Arthritis in Sprague Dawley Rat
Alluri V. Krishnaraju, Chundi B.M. Rao, Dodda Sundararaju, Krishanu Sengupta and Golakoti Trimurtulu
DOI : 10.3844/ajidsp.2009.68.73
American Journal of Infectious Diseases
Volume 5, Issue 2
Problem statement: Anti-inflammatory agents, particularly steroids and cyclooxygenase inhibitors are often associated with adverse side effects including, GI irritation, ulcers, hypertension and cardiac abnormalities. A safe and effective intervention is essential for the treatment of inflammatory disorders. Approach: Abstract Vitex leucoxylon L., a medicinal plant of the verbenaceae family, used in traditional medicine for relieving headache and catarrh. VL-89/185A, was obtained by bio activity-guided fractionation using 5-Lipoxygenase inhibitory activity. VL-89/185A was further tested against Freund's Complete Adjuvant (FCA) induced arthritis in Sprague Dawley (SD) rat. Results: Oral supplementation of VL-89/185A resulted in significant anti-inflammatory effects as indicated by reduction in paw edema both at 100 and 250 mg kg-1 doses when compared to untreated control rats. Furthermore, treatment with VL-89/185A significantly reduced circulating proinflammatory cytokines TNF-α and IL-1β. The safety of VL-89/185A was established (LD50 >5000 mg kg-1 body) by acute oral toxicity limit test according to OECD guidelines 425. Conclusion: The safety and efficacy profiles indicated that VL-89/185A is a safe intervention for inflammatory disorders.
© 2009 Alluri V. Krishnaraju, Chundi B.M. Rao, Dodda Sundararaju, Krishanu Sengupta and Golakoti Trimurtulu. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.