Target Identification in Ory S1 Pollen Protein Allergen from Oryza sativa in the Course of Construction of Hypoallergenic Vaccines
Ruchi Sharma and A. K. Singh
DOI : 10.3844/ajidsp.2009.142.147
American Journal of Infectious Diseases
Volume 5, Issue 2
Problem statement: Recombinant-based approaches are mostly focused on genetic modification of allergens to produce molecules with reduced allergenic activity and conserved antigenicity, such as hypoallergens. Recombinant allergens represent promising tools for diagnosis and therapy of type I allergy. This approach was probably feasible with every allergen with known amino acid sequence. Approach: The primary aim of this study was to determine the consensus epitope from twenty homologous protein sequences of Ory S1 allergenic protein sequence from Oryza sativa (indica group) pollen. Molecular modeling calculations had been used to investigate the allergenic protein models for the epitope. Results: Oryza sativa (japonica), Phleum pratense, Poa pratensis, Holcus lanatus, Lolium perenne, Triticum aestivum, Dactylis glomerata and Zea mays were found more closely related (alignment score 1145-812) among all the homologs and investigated further. The major binding pocket comprised an area of 604.5
© 2009 Ruchi Sharma and A. K. Singh. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.