Research Article Open Access

Patient and Mouse Antibodies against Dengue Virus Nonstructural Protein 1 Cross-React with Platelets and Cause Their Dysfunction or Depletion

Chiou-Feng Lin1, Huan-Yao Lei1, Ching-Chuan Liu1, Hsiao-Sheng Liu1, Trai-Ming Yeh1, Robert Anderson1 and Yee-Shin Lin1
  • 1 ,
American Journal of Infectious Diseases
Volume 4 No. 1, 2008, 69-75

DOI: https://doi.org/10.3844/ajidsp.2008.69.75

Submitted On: 9 August 2007
Published On: 31 March 2008

How to Cite: Lin, C., Lei, H., Liu, C., Liu, H., Yeh, T., Anderson, R. & Lin, Y. (2008). Patient and Mouse Antibodies against Dengue Virus Nonstructural Protein 1 Cross-React with Platelets and Cause Their Dysfunction or Depletion. American Journal of Infectious Diseases, 4(1), 69-75. https://doi.org/10.3844/ajidsp.2008.69.75

Abstract

Thrombocytopenia is a clinical manifestation in dengue virus (DV) infection, yet its pathogenic mechanisms are unresolved. We previously showed that dengue patient sera contained antibodies cross-reactive with platelets. In this study, we demonstrated that the anti-platelet activity of dengue patient sera was due to the antibodies against DV nonstructural protein 1 (NS1). Studies using DV-infected or recombinant NS1-immunized mouse sera showed that anti-NS1 antibodies cross-reacted with human platelets. The platelet-binding activity of dengue patient sera or anti-NS1 antibodies was inhibited by treatment of platelets with anti-NS1 or patient sera. Further investigation showed that anti-NS1 antibodies were able to inhibit platelet aggregation and cause platelet lysis in the presence of complement. The platelet-binding activity and the induction of platelet lysis mediated by dengue patient sera or anti-NS1 antibodies were increased when platelets were activated by ADP or thrombin. Taken together, anti-NS1 antibodies account for the cross-reactivity with platelets and cause platelet dysfunction or depletion, which may be involved in the pathogenesis of dengue diseases.

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Keywords

  • Autoantibody
  • dengue virus
  • platelet
  • NS1