Anti-Prm Antibody as an Autoantibody in Dengue Virus Infection
Kao-Jean Huang, Yu-Tien Cheng, Yee-Shin Lin, Jyh-Hsiung Huang, Hsiao-Sheng Liu, Trai-Ming Yeh, Ching-Chuan Liu and Huan-Yao Lei
DOI : 10.3844/ajidsp.2008.60.68
American Journal of Infectious Diseases
Volume 4, Issue 1
We have reported that anti-prM antibody is an enhancing antibody that enhances DV infection of non-Fc receptor bearing cells by dual specificity. We identified the epitope recognized by this anti-prM antibody, M3, which is located at the a.a.53-67of the prM protein nearby the prM/M cleavage junction and these anti-M3 antibodies could be detected in dengue patients sera. Surprisingly, this anti-prM antibody not only recognizes the prM protein of dengue virions, but also cross-reacts with epithelial cells, endothelial cells as well as T cells. The binding of anti-prM antibody to endothelial cells was dose-dependent and could be blocked by M3 peptides. Human M3-specific antibodies from dengue patient sera were demonstrated to be able to bind to endothelial cells and mediate ADE infection on K562 cells. Furthermore, the anti-prM antibody will mediate the antibody - dependent cell phagocytosis, leading to the similar severe form of DHF/DSS. In conclusion, anti-prM antibody plays two roles in the pathogenesis of dengue virus infection: to be an enhancing antibody and an autoantibody as well.
© 2008 Kao-Jean Huang, Yu-Tien Cheng, Yee-Shin Lin, Jyh-Hsiung Huang, Hsiao-Sheng Liu, Trai-Ming Yeh, Ching-Chuan Liu and Huan-Yao Lei. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.