American Journal of Biochemistry and Biotechnology

The Contamination Degree of Characteristic Fungi in Jet Fuel Detected by ATP Bioluminescence

Zezhen Li, Yun Xiong, Bo Chen and ZhenQiao Ma

DOI : 10.3844/ajbbsp.2018.162.167

American Journal of Biochemistry and Biotechnology

Volume 14, Issue 2

Pages 162-167

Abstract

Adenosine Triphosphate (ATP) lysate of the characteristic fungi in jet fuel was screened and three fluorescein-luciferase systems were compared in the paper. The correlation between ATP bioluminescence and traditional plate count for measuring the number of microorganism was also investigated and then a method of detecting the contamination degree of the characteristic fungi of jet fuel by ATP bioluminescence were initially established. The results show that the effect of ATP extraction taking surfactant Benzyldimethylhexadecylammonium chloride (BAC) as the microbial lysate was optimal and the optimal concentration and the action time were 0.15% and 30s respectively; The fluorescein-luciferase system after screening has a good detection limit, up to 10-15mol ATP; using ATP bioluminescence and traditional plate method to measure the microbial quantity, they have good correlation. Utilizing ATP bioluminescence to detect the contamination degree of the characteristic fungi in jet fuel can shorten the test time to 10min, which is suitable for rapid detection and has good application prospects.

Copyright

© 2018 Zezhen Li, Yun Xiong, Bo Chen and ZhenQiao Ma. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.