DNA-MARKERS OF SUNFLOWER RESISTANCE TO THE DOWNY MILDEW (PLASMOPARA HALSTEDII)
A. V. Usatov, A. I. Klimenko, K. V. Azarin, O. F. Gorbachenko, N. V. Markin, V. E. Tikhobaeva, Yu. A. Kolosov, O. A. Usatova, S. Yu. Bakoev, M. Yu. Bibov and L. V. Getmantseva
DOI : 10.3844/ajbbsp.2014.125.129
American Journal of Biochemistry and Biotechnology
Volume 10, Issue 2
One of the areas of biotechnology sunflower is the development and testing of DNA markers of important agronomic traits and in particular markers of resistance to downy mildew. Resistance of 16 Rf-lines of sunflower to the races 330 and 710 of Plasmopara halstedii has been studied. Genotyping of these lines was carried out using 9 STS-markers of three Pl-loci, Pl5, Pl6 and Pl8, associated with the resistance of sunflower to downy mildew. Only two out of nine STS-markers, HaP2 and HaP3 (locus Pl6), allowed us to identify the lines, which demonstrated resistance to the downy mildew under the conditions of artificial infection.
© 2014 A. V. Usatov, A. I. Klimenko, K. V. Azarin, O. F. Gorbachenko, N. V. Markin, V. E. Tikhobaeva, Yu. A. Kolosov, O. A. Usatova, S. Yu. Bakoev, M. Yu. Bibov and L. V. Getmantseva. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.