American Journal of Biochemistry and Biotechnology

PHENOLIC CONTENT, ANTIOXIDANT, ANTIMICROBIAL AND CYTOTOXIC ACTIVITIES OF ETHANOLIC EXTRACT OF SALIX ALBA

Ghassan Mohammad Sulaiman, Nehia Nima Hussien, Thorria Raddam Marzoog and Hussain Abood Awad

DOI : 10.3844/ajbbsp.2013.41.46

American Journal of Biochemistry and Biotechnology

Volume 9, Issue 1

Pages 41-46

Abstract

The total phenolic content, antioxidant, antimicrobial and cytotoxic activities of hot ethanolic extract of Salix alba bark were investigated. The antioxidant properties and the total phenolic contents of the extract were assessed by 1, 1-Diphenyl- 2-Picrylhydrazyl (DPPH) free radical scavenging and Folin-Ciocalteu methods, respectively. The extract showed significant antioxidant activity and antimicrobial activities against Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans. The highest effect was observed on C. albicans, followed by S. aureus then P. aeruginosa. While, Escherichia coli and Klebsiella pneumoniae were not affected. The cytoxicity of S. alba extract against the Human Leukemia (HL-60) cell line was evaluated, it had been noticed that the extract significantly reduced the viability of HL-60 cells in a dose- and time-dependent response relationship. Findings from the present study indicate that extract is highly cytotoxic to HL-60 cells. The antimicrobial and cytotoxic activities of S. alba extract seemed to be positively correlated with their antioxidant potentials.

Copyright

© 2013 Ghassan Mohammad Sulaiman, Nehia Nima Hussien, Thorria Raddam Marzoog and Hussain Abood Awad. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.