Isolation of the Antimicrobial Cyclic Peptide Subtilosin a from a Gut-Associated Bacillus subtilis Strain
Ghislain Schyns, Claudia R. Serra, Adriano O. Henriques, AnthonyArguelles-Arias , Bernard Joris and Patrick Fickers
DOI : 10.3844/ajbbsp.2013.307.317
American Journal of Biochemistry and Biotechnology
Volume 9, Issue 3
The endospore-forming Bacillus subtilis has been used as probiotics over the last 50 years. However, little is known on how Bacillus spp act in the gut compared to other well-characterized probiotics such as lactic acid bacteria. It is believed that the competitive exclusion of pathogens results from different mode of action notably the production of antimicrobial compounds such as bacteriocins. Here, we report the characterization of the unexpected ability of a gut-associated B. subtilis BSP1 to synthetize the cyclic bacteriocin subtilosin A at high level. Our findings suggest that the BSP1 phenotype could be related, at least in part, to a subsequent increased expression level of the subtilosin A biosynthetic gene cluster sbo-alb in response to a higher activity of the stationery and sporulation master regulator Spo0A.
© 2013 Ghislain Schyns, Claudia R. Serra, Adriano O. Henriques, AnthonyArguelles-Arias , Bernard Joris and Patrick Fickers. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.