Advances in Menaquinone-7 Production by Bacillus subtilis Natto: Fed-Batch Glycerol Addition
Aydin Berenjian, Raja Mahanama, Andrea Talbot, Hubert Regtop, John Kavanagh and Fariba Dehghani
DOI : 10.3844/ajbbsp.2012.105.110
American Journal of Biochemistry and Biotechnology
Volume 8, Issue 2
Menaquinone-7 was produced by fermentation mainly using Bacillus subtilis species. Due to the low concentration of menaquinone-7 in fermentation processes and the associated expense of recovering the menaquinone-7, it was desirable to improve the fermentation concentration. The aim of this study was to determine the effect of fed-batch glycerol addition in the production of menaquinone-7 during the fermentation of Bacillus subtilis natto in both small (25-mL) and bench scale (3-L) fermenters. Liquid phase fermentation was carried out aerobically at 40°C for a period of 6 days. The pH, bacterial growth, glycerol and menaquinone-7 concentrations were measured every day. Maximum menaquinone-7 was produced when 2% (w/v) glycerol was added to the fermentation media in the second day (48 h) of fermentation. The results were demonstrated 40% increase of menaquinone-7 concentration compared to batch culture. This study provides valuable data for further optimization and scale-up the menaquinone-7 fermentation process.
© 2012 Aydin Berenjian, Raja Mahanama, Andrea Talbot, Hubert Regtop, John Kavanagh and Fariba Dehghani. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.