ADVANCES IN MENAQUINONE-7 PRODUCTION BY BACILLUS SUBTILIS NATTO: FED-BATCH GLYCEROL ADDITION
Aydin Berenjian, Raja Mahanama, Andrea Talbot, Hubert Regtop, John Kavanagh and Fariba Dehghani
DOI : 10.3844/ajbbsp.2012.105.110
American Journal of Biochemistry and Biotechnology
Volume 8, Issue 2
Menaquinone-7 was produced by fermentation mainly using Bacillus subtilis species. Due to the low concentration of menaquinone-7 in fermentation processes and the associated expense of recovering the menaquinone-7, it was desirable to improve the fermentation concentration. The aim of this study was to determine the effect of fed-batch glycerol addition in the production of menaquinone-7 during the fermentation of Bacillus subtilis natto in both small (25-mL) and bench scale (3-L) fermenters. Liquid phase fermentation was carried out aerobically at 40°C for a period of 6 days. The pH, bacterial growth, glycerol and menaquinone-7 concentrations were measured every day. Maximum menaquinone-7 was produced when 2% (w/v) glycerol was added to the fermentation media in the second day (48 h) of fermentation. The results were demonstrated 40% increase of menaquinone-7 concentration compared to batch culture. This study provides valuable data for further optimization and scale-up the menaquinone-7 fermentation process.
Cite this Article
Berenjian, A., R. Mahanama, A. Talbot, H. Regtop and J. Kavanagh et al., 2012. Advances in menaquinone-7 production by Bacillus subtilis natto: Fed-batch glycerol addition. Am. J. Biochem. Biotechnol., 8: 105-110.