Uterus-Relaxing Study of a Sudanese Herb (El-Hazha)
Aimun Abdelgaffar Elhassan Ahmed, Robert Gaspar, Arpad Marki, Andrea Vasas, Mahmoud Mudawi Eltahir Mudawi, Judit Hohmann and George Falkay
DOI : 10.3844/ajbbsp.2010.231.238
American Journal of Biochemistry and Biotechnology
Volume 6, Issue 3
Problem statement: The aim of this study is to investigate the pharmacological effects of the Methanolic-extract (AH2) of El-Hazha and its sub-fractions. Approach: These investigations were carried out on in vitro isolated uterus preparations from Non-Pregnant (NP) and Late-Pregnant rats (LP). In parallel displacement radio-ligand binding assay was performed for β-Adrenergic Receptors (β-ADR). Results: Showed that the herb and its different fractions produced dose-dependent relaxant effect (p<0.05, t-test, n = 6) on uterine contraction elicit by 25 mM KCl in both NP and LP uteri that affected significantly by fractionation, however, the effect of the most active fraction (AH2-11) was reversed by prior addition of propranolol (non-specific β-antagonist), but not affected by progesterone pre treatment of the LP rats. In addition, AH2 only in high concentration displaced isotopes from β-ADR, this affinity changed markedly by fractionation. Conclusion: We validate the fractionation effect on its relaxant activity and found partial role for β-ADR on mediating this activity. Future study was recommended to isolate and investigate its active components to enhance this activity or to discover a new novel natural therapeutic agent(s).
© 2010 Aimun Abdelgaffar Elhassan Ahmed, Robert Gaspar, Arpad Marki, Andrea Vasas, Mahmoud Mudawi Eltahir Mudawi, Judit Hohmann and George Falkay. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.