An Optimized Medium for Screening of L-Asparaginase production by Escherichia coli
Younes Ghasemi, Alireza Ebrahiminezhad, Sara Rasoul-Amini, Gholamreza Zarrini, Mohammad Bagher Ghoshoon, Mohammad Javad Raee, Mohammad Hossein Morowvat, Farshid Kafilzadeh and Aboozar Kazemi
DOI : 10.3844/ajbbsp.2008.422.424
American Journal of Biochemistry and Biotechnology
Volume 4, Issue 4
Purified L-asparaginase II from Escherichia coli has been supplied and employed in the acute leukemia and other malignant neoplasms chemotherapy. L-asparaginase II gene (ansB) in E. coli is under regulation and certain conditions is needed for expression of this gene. In this investigation ,the various concentrations of modified M9 medium ingredients and various carbon source were tested to optimize the medium for expression and identification of L-asparaginase in E. coli. Finally a semi-quantitative plate assay for L-asparaginase producing Escherichia coli is reported.
© 2008 Younes Ghasemi, Alireza Ebrahiminezhad, Sara Rasoul-Amini, Gholamreza Zarrini, Mohammad Bagher Ghoshoon, Mohammad Javad Raee, Mohammad Hossein Morowvat, Farshid Kafilzadeh and Aboozar Kazemi. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.