Mutations in Tumor suppressor TP53 Gene in Formalin- Fixed, Paraffin Embedded Tissues of Squamous Cell Carcinoma (SCC) of Lung Cancer
Abbas Mohammadi, Ali Vaziri Gohar and Mohamad Reza Shakibaie
DOI : 10.3844/ajbbsp.2008.1.6
American Journal of Biochemistry and Biotechnology
Volume 4, Issue 1
The TP53 gene mutations have been extensively studied in lung cancer and have been used as a means to understand the origin(s) and mechanisms of these mutations in lung cancer development. TP53 gene mutations occur frequently in many human cancers. Analysis of TP53 gene mutations can also provide clues to the etiology of tumor formation. Formalin-fixed, paraffin embedded (FFPE) tissues from patients undergone surgery between 1997 to 2005 were provided the pathology department of Afzalipour Hospital, Kerman, Iran. The mutational status of the exons 5 & 8 of the TP53 gene was screened by PCR followed by sequencing. Of the 25 patient samples sent for sequencing, 22 produced usable results for exon 5 and of these, 18 cases (81.8%) showed mutations. 18 samples also produced usable results for exon 8, and of these, 15 (83.3%) cases contained mutations. We identified a total of 113 mutations in tumor suppressor TP53 gene of squamous cell carcinoma of lung tumors. At least 69 mutations were found in Exon 5 (in 18 cases) and 44 mutations in Exon 8 (in 15 cases). The most frequent mutations in exon 5 were deletion, G to T transvertion, G to A transition, G to C transversion and in exon 8 were A to T and G to T tansversion and deletion. Mutations were spread throughout the exons 5 & 8 but showed a strong preference for some mutational hotspots. These findings showe that TP53 gene mutations contribute to the pathogenesis of non-small cell lung cancer significantly and the frequency of the mutations was higher in Iran compared to other areas described in the literature.
© 2008 Abbas Mohammadi, Ali Vaziri Gohar and Mohamad Reza Shakibaie. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.