Optimization of Conditions for Extraction of Pepsin-Soluble Collagen from Indonesian Local “Kacang” Goatskin by Response Surface Methodology
Rina Wahyuningsih, Rusman , Nurliyani , Ambar Pertiwiningrum, Abdul Rohman, Nanung Agus Fitriyanto and Yuny Erwanto
DOI : 10.3844/ajavsp.2018.70.75
American Journal of Animal and Veterinary Sciences
Volume 13, Issue 2
Indonesian local “Kacang” goat is an animal species widely known by consumers over the world which possesses many health effects. Goatskin is one of body parts which has potential as collagen sources. The optimal conditions for collagen extraction were determined by response surface methodology in this study. Multiple response optimization designs were applied to evaluate the effects of two independent variables (curing time of NaOH and hydrolysis time) on the pepsin-soluble collagen yield of Indonesian local “Kacang” goatskin. Correlation analysis of the mathematical regression model indicated that quadratic polynomial model could be employed to optimize the extraction of pepsin-soluble collagen. The optimal conditions to obtain the highest yield of pepsin-soluble collagen was without curing NaOH and 24 h time of hydrolysis.
© 2018 Rina Wahyuningsih, Rusman , Nurliyani , Ambar Pertiwiningrum, Abdul Rohman, Nanung Agus Fitriyanto and Yuny Erwanto. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.