American Journal of Animal and Veterinary Sciences

Tumor Suppressor P53 Gene Mutations Pattern Induced by Repeated Fried Palm Oil in Rats

Abeer A.I. Hassanin, Abeer G.A. Hassan, Saadia A. Ali and Takao Itakura

DOI : 10.3844/ajavsp.2014.230.238

American Journal of Animal and Veterinary Sciences

Volume 9, Issue 4

Pages 230-238

Abstract

The present study aimed to examine the presence of p53 gene mutations in white albino rats fed on repeated fried palm oil. Eighteen sexually mature male Albino rats were used throughout the experiment. Rats were divided randomly into three groups, 6 animals each, namely; control group (CO) which orally administered by distilled water (NO) group which fed on basal diet containing fresh pure palm oil (FO) group which fed on the basal diet containing repeated fried palm oil with a dose of 150 mL kg-1 diet. At the end of the experiment, liver samples were taken, kept at -80°C for genetic alteration studies. Results shows the presence of fifty eight base-pair substitutions mutations which include a total of twelve base pair substitutions arose at A:T base pairs, fourteen base pair substitutions arose at G:C base pairs, Eighteen base pair substitutions at T: A base pairs, fourteen base pair substitutions arose at C:G base pairs. Of the fifty eight substitutional mutations recorded, there were thirty one silent (same sense) mutations and twenty seven missense mutations which causing the substitution of amino acids.

Copyright

© 2014 Abeer A.I. Hassanin, Abeer G.A. Hassan, Saadia A. Ali and Takao Itakura. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.