The Ovario-Protective Effect of Erythropoietin against Oxidative Damage Associated with Reperfusion Following Ovarian Torsion in Rat
Sayyah-Melli Manizheh, Kazemi-Shishvan Maryamalsadat, Solaimani-Rad Jafar, Rashidi Mohammad-Reza, Roshangar Leila, Rashtchizadeh Nadereh, Ouladesahebmadarek Elaheh, Ghojazadeh Morteza and Mashrabi Omid
DOI : 10.3844/ajavsp.2011.18.24
American Journal of Animal and Veterinary Sciences
Volume 6, Issue 1
Problem statement: To show the effect of recombinant Erythropoietin (rhEPO) on the ovarian viability and histology in the twisted ischemic ovaries in rats, followed by detorsion. Approach: An experimental study was conducted in primate clinic of the School of Medicine, Tabriz University of Medical Sciences from Dec. 2008 to Apr. 2009. Forty, 4 month old Wistar rats are cased in the present study divided into 4 groups. Ovarian ischemia was performed by torsion which was kept stable by using a vascular microclip for 4 h. In group 1, the ovary were surgically removed, fixed and analyzed histochemically. In group 2, the same procedure was repeated after 3 h reperfusion. In the next two groups, the same was performed rhEPO was administrated 400 u kg−1 1 h after torsion of the ovaries. Results: Thirty-two out of 40 rats were followed. There was a significant difference between groups in the levels of Malondialdehyde (MDA), Total Antioxidants (TA), Superoxidase (SOD), Nitric Oxide (NO) glutathione ( p<0.001). Addition of rhEPO maintained the normal histologic appearance in interventional groups compared with controls. Conclusion: Administration of rhEPO was effective in reducing the ischemic effect and free radicals damages of ovarian torsion in rats.
© 2011 Sayyah-Melli Manizheh, Kazemi-Shishvan Maryamalsadat, Solaimani-Rad Jafar, Rashidi Mohammad-Reza, Roshangar Leila, Rashtchizadeh Nadereh, Ouladesahebmadarek Elaheh, Ghojazadeh Morteza and Mashrabi Omid. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.