American Journal of Animal and Veterinary Sciences

Effect of Equilibration Temperature on In vitro Viability and Subsequent Embryo Development of Vitrified-Warmed Immature Bovine Oocytes

Hajarian Hadi, H. Wahid, O. A. Mazni, Y. Rosnina, M. Daliri, M. Dashtizad, A. Faizah, K. C. Yap, F. J. Fahrul and A. Fazly

DOI : 10.3844/ajavsp.2010.71.75

American Journal of Animal and Veterinary Sciences

Volume 5, Issue 2

Pages 71-75

Abstract

Problem statement: Vitrification is replacing conventional slow freezing to cryopreserve gametes and embryos especially for in vitro production of embryo in domestic animal species. However, the results are still not satisfactory. The aim of this experiment was to study the effect of different equilibration temperatures on in vitro viability of immature bovine oocytes after vitrification. Approach: Oocytes were obtained from slaughterhouse ovaries. Only grade one oocytes were used. Oocytes were equilibrated in three different temperatures: 32, 37, or 41

Copyright

© 2010 Hajarian Hadi, H. Wahid, O. A. Mazni, Y. Rosnina, M. Daliri, M. Dashtizad, A. Faizah, K. C. Yap, F. J. Fahrul and A. Fazly. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.