American Journal of Animal and Veterinary Sciences

Synergistic Effect of Insulin on in vitro Development of Immature Bovine Oocytes

Mojtaba Dashtizad, Abd W. Haron, Rosnina Yusoff, Morteza Daliri, Hadi Hajarian, Mehdi Najari, Yap K. Chee and Abas M. Othman

DOI : 10.3844/ajavsp.2010.258.265

American Journal of Animal and Veterinary Sciences

Volume 5, Issue 4

Pages 258-265

Abstract

Problem statement: Development of efficient culture system to support embryonic development would be valuable when quality of produced embryos was important. However, the rate of bovine embryo production in vitro was still lower than expected. Present study, including of three experiments, was carried out to investigate the effect of insulin on nuclear maturation and subsequent development of immature bovine oocytes and in vitro fertilized embryos. Approach: Grade one cumulus-oocyte-complexes harvested from slaughterhouse ovaries were selected and randomly allocated in each treatment groups. In experiment 1, in vitro maturation medium (Hepes-buffered medium 199 + fetal calf serum + gonadotrophins + antibiotics) supplemented with 0 (control), 1, 10, 20 and 100

Copyright

© 2010 Mojtaba Dashtizad, Abd W. Haron, Rosnina Yusoff, Morteza Daliri, Hadi Hajarian, Mehdi Najari, Yap K. Chee and Abas M. Othman. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.