American Journal of Animal and Veterinary Sciences

Effect of Age and Postmortem Time on Some White-Tailed Deer (Odocoileus virginianus texanus) Epididymal Sperm Characteristics and Response of Cryopreservation

J. Ake-Lopez, E. Cavazos-Arizpe, J. G. Magana-Monforte, F. Centurion-Castro and C. Silva-Mena

DOI : 10.3844/ajavsp.2010.183.186

American Journal of Animal and Veterinary Sciences

Volume 5, Issue 3

Pages 183-186


Problem statement: Males rather than females of the White-Tailed deer (Odocoileus virginianus texanus) are more susceptible to hunting because their physical characteristics, therefore their elimination can increase a genetic degradation and a lower productivity because of the effect of gender. Thus, the aim of the current study was to evaluate the effect of age and postmortem time of White-Tailed deer on its epididymal-tail sperm motility and morphology and the response to sperm cryopreservation. Approach: Twenty two hunted deer were used and were classified into three age-groups: A = 1.5-3.5; B = 4.5-5.5 and C = 6.5-7.5 years old and four groups according to postmortem time: 1= 0-3; 2 = >3-5; 3 = >5-7 and 4 = > 7 h. Two samples per animal (one per each epididymal) were diluted and frozen with Triladyl® and Tris-Fructose. Thawing was done 72 h post freezing. Results: Age did not neither affect motility nor morphology (p>0.05). Postmortem time had a deleterious effect on motility (p<0.05), a significant drop in this trait was found after 5 h postmortem (58.1, 56.1, 37.5 and 38.3% motility in groups 1-4, respectively). However, did not affect morphology until 7 h postmortem and after 7 h an unexpected, significant (p<0.05) improvement was found (66.4, 74.9, 62.2 and 83.8% of normal sperm in groups 1-4. As regarding freezing ability, though without a statistical difference (p>0.05), the percentage of samples with acceptable motility after freezing and thawing was greater with Tris-Fructose than with Triladyl (36 Vs 18%). Conclusion: It was concluded that these results indicate that age as studied does not affect epididymal sperm quality in this species, while post-mortem time has a detrimental effect on motility and epididymal sperm can be successfully cryopreserved, for which Tris-Fructose would be better as an extender than Triladyl.


© 2010 J. Ake-Lopez, E. Cavazos-Arizpe, J. G. Magana-Monforte, F. Centurion-Castro and C. Silva-Mena. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.