Isolation and Identification of Epiphytic Lactic Acid Bacteria from Guinea Grass (Panicum maximum)
M. Pasebani, H. Yaakub, K. Sijam, A. R. Alimon, M. Pasebani, H. Yaakub, K. Sijam and A. R. Alimon
DOI : 10.3844/ajavsp.2010.146.150
American Journal of Animal and Veterinary Sciences
Volume 5, Issue 2
Problem statement: Bacteria can perform a variety of beneficial functions, for example many lactic acid bacteria are responsible for fermentation of silage in the process of forage conservation. In the making of silage, epiphytic lactic acid bacteria are usually insufficient in numbers to promote efficient lactate fermentation. This study was conducted to identify the predominant indigenous bacteria, with emphasis on lactic acid bacteria, from Guinea grass (Panicum maximum). Approach: Two different condition of growth using nutrient and MRS agar were prepared for isolation of the bacteria. In total, 18 purified isolates were identified by BIOLOG identification system which comprised of 9 bacterial species. Standard plate count in the both conditions was considered. Results: Three bacterial species based on the first condition of growth were identified which were belonging to Flavimonas oryzihabitans, Enerobacter cloacae, Sphingomonas paucimobilis B. Lactic acid bacteria based on the second condition of growth were belonging to Weissella confusa, Weissella paramesenteroides, Leuconostoc mesenteroides ssp. dextranicum, Lactococcus lactis ssp. hordniae. Result of plate count showed that 8.3
© 2010 M. Pasebani, H. Yaakub, K. Sijam, A. R. Alimon, M. Pasebani, H. Yaakub, K. Sijam and A. R. Alimon. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.