Reactive Nitrogen Intermediates Production by Macrophages of Mycobacterium bovis-Infected Goats and Supplemented with Dyhidroxyvitamin D3 in vivo
Aurora Martinez-Romero, Jose Luis Ortega-Sanchez, Jose Ramon Hernandez-Salgado, Margarita Yolanda Mendoza-Ramos, Rafael Rodriguez-Martinez, Maribel Cervantes-Flores, Joel Lopez-Perez, Norma Urtiz-Estrada, Miguel Arenas-Vargas and Raul Villegas-Viscaino
DOI : 10.3844/ajavsp.2009.6.13
American Journal of Animal and Veterinary Sciences
Volume 4, Issue 1
Problem statement: Tuberculosis (TB) remains one of the world's major health problems. To evaluate in vivo indices of cellular sensitization, antigen-induced Reactive Nitrogen Intermediates (RNI) responses by blood mononuclear cells from Mycobacterium bovis BCG-infected goats supplemented with 1, 25 dyhidroxyvitamin D3 [1, 25-(OH)2D3]. Approach: An experimental, longitudinal and comparative study was planned. Twelve animals of goat cattle 20-to 24-month-old sannen selected themselves. Five samplings were made, previous to the inoculation (zero d), 3, 7, 14 and 21 d after applying the treatments. The mononuclear cells by the Ficoll-hypaque method were obtained. The RNI, nitrites and nitrates (NO2- and NO3-) were quantified by Enzyme-Linked Immunosorbent Assay (ELISA). Results: The treatment with the 1, 25(OH)2D3 stimulated the NO3- synthesis indicating, that by itself it is a good modulador of the micobacterial replication and in the treatment with M. bovis-BCG vaccine increased as a result to the treatment with 1,25(OH)2D3. The exhibition to M. bovis-BCG vaccine with the treatment with 1, 25(OH)2D3 was able to increase answer NO3- in exposed animals. Conclusion: The 1, 25(OH)2D3 stimulated in vivo the production of RNI in goats exposed to M. bovis BCG vaccine.
© 2009 Aurora Martinez-Romero, Jose Luis Ortega-Sanchez, Jose Ramon Hernandez-Salgado, Margarita Yolanda Mendoza-Ramos, Rafael Rodriguez-Martinez, Maribel Cervantes-Flores, Joel Lopez-Perez, Norma Urtiz-Estrada, Miguel Arenas-Vargas and Raul Villegas-Viscaino. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.