Chromium Picolinate did not Effect on the Proliferation and Differentiation of Myoblasts
M.C. Tsa and T.F. Lien
DOI : 10.3844/ajavsp.2007.79.83
American Journal of Animal and Veterinary Sciences
Volume 2, Issue 4
This experiment is conducted in vitro to investigate trivalent chromium picolinate affects the proliferation and differentiation of myoblasts. A myoblasts cell line (C2C12) from rats was used in the experiment. These were randomly divided into the control group, the Pic group (50ppb picolinate) and the CrPic group (50ppb chromium picolinate). The differentiation of myoblasts reveals that the number of differentiated myotubes, creatine kinase (CK) activity and the aldolase (ALB) activity do not differ among the three groups (P > 0.05). The activity of hexokinase in the CrPic and Pic groups clearly exceeds that in the control group (P < 0.05), and the activity of myokinase in the Pic group exceeds that in the CrPic and control groups (P < 0.05). Besides, the transcription factor myogenin exerts no effect on any of the three groups (P > 0.05). Myoblast proliferation was the same across the three groups (P > 0.05), and the quantity of DNA in the control group exceeded that in the Pic group (P < 0.05). The experiment indicated that 200ppb chromium picolinate did not influence the proliferation and differentiation of myoblasts.
© 2007 M.C. Tsa and T.F. Lien. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.