Enzymatic Kinetics of Fatty Acids Methyl Ester from Kapok Seed Oil (Ceiba Petandra) Using Lipozyme TL IM | Science Publications

American Journal of Applied Sciences

Enzymatic Kinetics of Fatty Acids Methyl Ester from Kapok Seed Oil (Ceiba Petandra) Using Lipozyme TL IM

Erin Ryantin Gunawan, Dedy Suhendra and Baiq Rizkia Miftahatul Hasanah

DOI : 10.3844/ajassp.2017.1146.1151

American Journal of Applied Sciences

Volume 14, Issue 12

Pages 1146-1151

Abstract

Synthesis of Fatty Acids Methyl Ester (FAME) utilizing oil extracted from kapok seeds (Ceiba petandra) and catalyzed by lipozyme TL. 1M via enzymatic kinetics transesterification was succesfully conducted A mixture of different FAME, widely known as biodiesel is one of the sources of renewable energy. A kinetic analysis was investigated to identify the reaction model and the rate equation and to calculate kinetic parameters. The result obtained showed that the enzymatic kinetics followed a Michaelis-Menten model. The transesterification kinetic followed the Ping-pong Bi-Bi mechanism characterized by the υmax, Km(KO), Km(Met) values of 3.06×10-5 mmol/min.mg catalyst, 0.95×102 ppm, 1.30×102 ppm, respectively.

Copyright

© 2017 Erin Ryantin Gunawan, Dedy Suhendra and Baiq Rizkia Miftahatul Hasanah. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.