EFFECT OF PIOGLITAZONE ON HEPATIC ULTRA-STRUCTURE AND METABOLIC CHANGES INDUCED BY A HIGH SUCROSE DIET IN RATS
- 1 King Khalid University, Egypt
- 2 , Saudi Arabia
- 3 Benha University, Egypt
- 4 King Khalid University, Saudi Arabia
Copyright: © 2020 Mohamed D. Morsy, Ahmed S. Khashaba, Ahmed I. Agamy and Refaat A. Eid. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Pioglitazone (Pio) is a PPAR-γ agonist insulin sensitizer has anti-inflammatory activity. Our novel aspect was to investigate its hepatic anti-inflammatory activity at the level of ultra-structure and enzymatic changes in a high sucrose diet animal model. Forty male Sprague Dawley rats were divided into four equal groups: Control; control Pio; high sucrose diet; high sucrose diet Pio treated groups. Fourteen weeks later, serum glucose, insulin, lipogram, gamma glutamyle transferase, alanine transaminase, aspartate transaminase, alkaline phosphatase, fetuin-A and adiponectin levels were measured. Hepatic tissue homogenate levels of tumor necrosis factor-α, interleukin-6 and myeloperoxidase activity were determined. Microscopic and ultra-structure hepatic changes were conducted in all animal groups. Administration of Pio in HS+Pio group reduced significantly the hepatic inflammatory markers and the hepatic enzymes compared with HS group. Both light and electron microscopic examination revealed a great improvement of the hepatic tissue with Pio treatment. This study suggested that Pio treatment in obesity; in addition to insulin sensitizing activity; could protect the liver from the development of hepatic inflammation induced by a high sucrose diet not only at the enzymatic but also at ultra-structure levels.
- Chronic Hepatic Inflammation
- High Sucrose Diet
- Hepatic Ultra-Structure Changes