American Journal of Applied Sciences

Simultaneous Determination of Amlodipine and Atorvastatin in Caduet® Tablets Using HPLC

Maha Abdalla Alnowaiser

DOI : 10.3844/ajassp.2013.849.852

American Journal of Applied Sciences

Volume 10, Issue 8

Pages 849-852

Abstract

A simple, selective, sensitive and precise, simultaneous high performance liquid chromatographic analysis of tablets containing amlodipine and atorvastatin was described. Good chromatographic separation was achieved using a Perfectsilr Target ODS-3 (4.6 cm × 250 mm, 5 µm) and a mobile phase consisting of acetonitrile-phosphate buffer pH 4.5 (55:45, v/v) at a flow rate 1ML min-1. The ultraviolet detector was set at wavelength 237 nm. Amlodipine and atorvastatin were measured at 1.071 and 3.765 min, respectively. The linear ranges for amlodipine and atorvastatin were 1-10 and 5-50 µg mL-1, respectively. The recoveries of amlodipine and atorvastatin in pharmaceutical preparation were all greater than 98.5% and their relative standard deviations were less than 2.0%. The limits of detection were 0.19 and 1.25 µg mL-1 for amlodipine and atorvastatin, respectively.

Copyright

© 2013 Maha Abdalla Alnowaiser. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.