Metabolic Fingerprinting of Three Malaysian Ginger (Zingiber officinale Roscoe) Using Gas Chromatography-Mass Spectromertry
H.J. Mahdi, R. Andayani and Ishak
DOI : 10.3844/ajassp.2010.17.23
American Journal of Applied Sciences
Volume 7, Issue 1
Problem statement: There is a chemical variation among different ginger cultivars detected by previous studies including the volatile and non-volatile oleo-resins. In this study we try to determine whether these variations are related to a genetic factor rather than environmental or intrinsic factors. Approach: A comprehensive metabolic fingerprinting from the leaves of three micro-propagated ginger cultivars Bukit Tinggi, Tanjung Sepat and Sabah was performed using a Gas Chromatography-Mass Spectrometry (GC-MS). Constituents of the ginger leaves were first extracted and then fractionated into methanolic and chloroform. The samples were subsequently methoximated and silylated prior to GC-MS analysis. Results: By applying this technique, we detected more than 300 compounds (polar and non-polar) in total originated from each ginger cultivar. Based on the GC-MS fragmentation, three different classes of metabolites were detected from the ginger cultivars, namely amino acids, carbohydrates and organic acids. A qualitative variation on the type of ginger metabolites was observed, albeit no marked different found in the level of the metabolites. Conclusion: Apparently the chemical variations among the three ginger cultivars were due to genetic effects since almost all other environmental and intrinsic factors were eliminated.
© 2010 H.J. Mahdi, R. Andayani and Ishak . This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.