Assessing of α-Amylase Activity of Midgut in Wheat Bug Eurygaster maura
Mohammad Mehrabadi and Ali R. Bandani
DOI : 10.3844/ajassp.2009.478.483
American Journal of Applied Sciences
Volume 6, Issue 3
To determine of α-amylase activity in the midgut of Eurygaster maura, some of adult insect was collected and their gut isolated and characterized. Enzyme samples from midguts of adults were prepared by the method of Cohen with slight modifications. α-Amylase activity was assayed based on Bernfeld method by the dinitrosalicylic acid (DNS) procedure. The activity of α-amylase in midgut was 0.083 U/insect. The optimum pH and temperature for the enzyme activity was determined to be 6-6.5 and 30-35°C, respectively. The enzyme activity was inhibited by addition of EDTA (Ethylenediamine tetraacetic acid) urea, CaCl2, MgCl2 and SDS but Mg2+, NaCl and KCl enhanced enzyme activity.
© 2009 Mohammad Mehrabadi and Ali R. Bandani. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.