Screening Three Strains of Pseudomonas aeruginosa: Prediction of Biosurfactant-Producer Strain
Gholamreza Dehghan-Noudeh, Mohammad Hasan Moshafi, Effat Behravan, Saeed Torkzadeh and Masoud Ahmadi Afzadi
DOI : 10.3844/ajassp.2009.1453.1457
American Journal of Applied Sciences
Volume 6, Issue 8
Problem statement: The chemical surfactants have some disadvantages; especially, toxicity and no biodegradability. Approach: Biosurfactants were the structurally diverse group of surface-active molecules synthesize by micro-organisms. The microbial surfactants were interesting, because of the biodegradable and have many applications in industry, agriculture, medicine. Results: In the present study, the production of biosurfactant by three strains of Pseudomonas aeruginosa (PTCC 1074, 1310 and 1430) was investigated. The hemolytic and foam forming activity of different strains were studied and consequently, P. aeruginosa PTCC 1074 was selected as the suitable strain. P. aeruginosa PTCC 1074 was grown in the nutrient broth medium and biosurfactant production was evaluated every 24 h by emulsification index and surface tension for the best of production time. After that, in order to get maximum production of biosurfactant, the selected strain was grown with different additives in nutrient broth and the best culture medium was found. The biosurfactant was isolated from the supernatant and its amphipathic structure was confirmed by chemical methods. Conclusion: Biosurfactant produced by Pseudomonas aeruginosa PTCC 1074 would be considered as a suitable surfactant in industries due to its low toxicity.
© 2009 Gholamreza Dehghan-Noudeh, Mohammad Hasan Moshafi, Effat Behravan, Saeed Torkzadeh and Masoud Ahmadi Afzadi. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.