Validation of SSR markers associated with Submergence Tolerance in Rice (Oryza sativa L.)
Kirill V. Azarin, Alexander V. Usatov, Andrey V. Alabushev, Pavel I. Kostylev, Maxim S. Makarenko and Aleksey A. Kovalevich
DOI : 10.3844/ajabssp.2016.142.147
American Journal of Agricultural and Biological Sciences
Volume 11, Issue 4
The present work is devoted to the validation of SSR markers associated with submergence tolerance (Sub1A) QTLs in rice. It was shown that only microsatellite markers RM 7481 differentiates donor lines and recipient variety. Based upon the results of DNA analysis with the marker of the RM 7481 of the F2-plants, the 14 homozygotes by the locus Sub1A, 40 samples carried Sub1A in the heterozygous state, 22 plants inherited only alleles from the recipient variety Novator were identified. Survival analysis of parental and hybrid plants (21 days after germination) showed that the most submergence tolerance forms were donor lines of Sub1A loci and F2-plants carrying Sub1A locus in homozygous and heterozygous state according to the data of molecular genetic analysis. Thus, it was shown that RM 7481 microsatellite marker is effective for breeding of submergence tolerance rice using Russian elite cultivars.
© 2016 Kirill V. Azarin, Alexander V. Usatov, Andrey V. Alabushev, Pavel I. Kostylev, Maxim S. Makarenko and Aleksey A. Kovalevich. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.