COMPARISON OF EDTA AND EDDS ENHANCTED PHYTOEXTRACTION OF Cr AND Pb FROM CONTAMINATED SOIL BY ANANAS COMOSUS (L.) MERR.
Pantawat Sampanpanish and Tippawan Pojanaporn
DOI : 10.3844/ajabssp.2014.361.368
American Journal of Agricultural and Biological Sciences
Volume 9, Issue 3
The effects of chelating agents on Chromium (Cr) and lead (Pb) absorption were studied by planting pineapple, Ananas comosus (L.) Merr. in contaminated soil. All plant samples were grown in a nursery for 30 days and then separated into seven sets: Set (1) had nothing added (Blank); (2) had Pb added as Pb(II) Nitrate (Pb(NO3)2) at 500 mg kg-1 soil; (3) added Pb(II) nitrate and EDTA, a chelating agent; (4) contained both Pb(II) nitrate and EDDS, a second chelating agent; (5) only added Cr as potassium dichromate (K2Cr2O7), at 400 mg kg-1 soil; (6) was treated with Cr (potassium dichromate) plus EDTA; and (7) contained both Cr (potassium dichromate) and EDDS. The chelating agent concentrations were 2 millimoles per kilogram soil. Soil and plant contamination levels were measured by analyzing the Cr, Cr(VI) and total Pb after growing for 30, 60, 90 and 120 days. The analysis divided the plant samples into two parts: Aboveground and underground. Plant growth was also analyzed by dry weight, root length and expression of toxicity through withered leaves and yellow leaf symptoms. The results of this study indicate that after 60 days, the EDTA agent had the highest Pb absorption efficiency, with the plant sample absorbing 288.14 mg Pb per kg soil in the aboveground part and 796.66 mg kg-1 soil in the underground part. The EDTA agent had high Cr absorption efficiency, with the plant sample absorbing Cr at 545.72 mg kg-1 soil in the aboveground part and 2267.99 mg kg-1 soil in the underground part after 90 days. The EDTA and EDDS agents did not affect pineapple growth and expression of toxicity symptoms were statistically significant (p≤0.05) compared with the control sets.
© 2014 Pantawat Sampanpanish and Tippawan Pojanaporn. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.