American Journal of Agricultural and Biological Sciences

Attenuated Total Reflectance-Fourier-Transform Infrared Microspectroscopy a Rapid Method for Microbial Strain Characterization

Meshref Awad Alruwaili

DOI : 10.3844/ajabssp.2013.135.141

American Journal of Agricultural and Biological Sciences

Volume 8, Issue 2

Pages 135-141

Abstract

Fourier-Transform Infrared (FTIR) spectroscopy and BHI 50CH were used to identify bacteria of the Lactobacillus (L.) at the species level. A previously developed method for measuring FTIR spectra and a strategy for their analysis provided the basis for selecting the FTIR spectra of reference bacterial strains and created a spectral library. The research was launched in which the spectra collected in the above library were used for developing a spectral reference for known reference bacterial strains and the practical value of the generated library was verified based on the results of identification of four bacterial strains viz. L. plantarum, L. casei, L. lactis and L. fermentum of known taxonomy as well as identification of 15 bacterial strains isolated from rumen extracts and identified on the basis of their taxonomy and biochemical tests. The application of prepared lactic acid bacteria reference library for analysis of advanced analysis of FTIR was provided an accurate identification of 90% of bacterial strains of the genus Lactic acid bacteria identified by FTIR-microspectroscopy.

Copyright

© 2013 Meshref Awad Alruwaili. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.