American Journal of Agricultural and Biological Sciences

Inulinase Production by Penicillium citrinum ESS in Submerged and Solid-State Cultures

Cynthia Lafuente-Castaneda, Gabriela Martinez, Juan C. Contreras-Esquivel, Raul Rodriguez-Herrera, Arely Prado-Barragan and Cristobal N. Aguilar

DOI : 10.3844/ajabssp.2012.129.134

American Journal of Agricultural and Biological Sciences

Volume 7, Issue 2

Pages 129-134

Abstract

Problem statement: This work reports the Penicillium citrinum ESS strain as a new source of inulinase (2,1-β-D fructanhydrolase, E.C. 3.2.1.7). Approach: The enzyme was produced in both, Solid-State (SSC) and Submerged Culture (SmC) using a basal medium added either with inulin or sucrose (5 gL-1). Results: The culture grew faster in SmC than in SSC with both carbon sources; however, yields of inulinase activity per gram of biomass were higher in SSC (1,658,237.28 for inulin and 66598.96 for sucrose) than in SmC (441.05 for inulin and 183.91 for sucrose). Conclusion: The highest inulinase production was obtained by SSC, either with inulin or sucrose, reaching values of 6650 and 2970 U/L respectively. In contrast, in SmC were obtained higher levels of inulinase activity (2241 U/L) with sucrose as carbon source (24 h), while with inulin, the enzyme production was negatively affected and retarded.

Copyright

© 2012 Cynthia Lafuente-Castaneda, Gabriela Martinez, Juan C. Contreras-Esquivel, Raul Rodriguez-Herrera, Arely Prado-Barragan and Cristobal N. Aguilar. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.