Enzymatic Bioconversion of Agave Leaves FiberHydrolysis Using Plackett-Burman Design
Miguel A. Medina-Morales, J. C. Contreras-Esquivel, H. De la Garza-Toledo, R. Rodriguez and Cristobal N. Aguilar
DOI : 10.3844/ajabssp.2011.480.485
American Journal of Agricultural and Biological Sciences
Volume 6, Issue 4
Problem statement: Biofuels production is becoming a key factor to help decrease pollution levels and the dependency of fossil fuels. Cellulose from lignocellulosic biomass is being used as a source of raw material for biofuels production, specifically bioethanol, so many ways to use it for this purpose are being developed. Approach: Cellulose content and enzymatic depolymerization of cellulose was evaluated in this contribution. Results: Cellulose content was of 67% on fibers, which places this material as a potential raw material for bioethanol production 42% of the cellulose content of the Agave leaves fibers was released as glucose due to enzymatic degradation. Seeing the behavior of the enzymatic hydrolysis at 96 h a mathematical model was applied which gave a time for enzymatic hydrolysis which must result in the maximum of glucose liberated under the conditions used for the process. Conclusion: Using Agave Atrovirens at 44 h of enzymatic hydrolysis will provide the highest yield of glucose which can be used for other processes such as ethanolic fermentation.
© 2011 Miguel A. Medina-Morales, J. C. Contreras-Esquivel, H. De la Garza-Toledo, R. Rodriguez and Cristobal N. Aguilar. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.