American Journal of Agricultural and Biological Sciences

Antagonist capacity of Newly Isolated Strains of Pseudomonas Fluorescens against Three Important Phytopathogenic Bacteria

Reynaldo De La Cruz-Quiroz, Catalina Chavez, Marcela Hernandez, Raul Rodriguez, Daniel Hernandez and Cristobal Noe Aguilar

DOI : 10.3844/ajabssp.2011.267.272

American Journal of Agricultural and Biological Sciences

Volume 6, Issue 2

Pages 267-272

Abstract

Problem Statement: Phytopatogenic bacteria cause several damages to plants with important economical consequences. They provoke losses of product quality affecting all commercial chain of crops, for this reason, their control is a priority. Approach: We evaluated antagonist capacity of newly isolated Pseudonomas fluorescens strains against three important phytopatogenic bacteria (Clavibacter michiganensis, Xanthomonas axonopodis and Erwinia carotovora). Soils from commercial cropping of Capsicum annum L of several Mexican regions were used to isolate P. fluorescens strains. Results: Isolates producing flourescein were purified on King B agar and biochemically identified. Crude extracts with and without cells were produced in King B broths and their antagonist capacities were evaluated by the plate diffusion procedure on nutritive agar. Conclusion: Obtained results demonstrated that cell free extracts exhibited a limited antagonist capacity in comparison of those extracts with cells, which showed an excellent capacity to inhibit the growth of C. michiganensis, X. axonopodis and E. carotovora, demonstrating the intracellular nature of the bioactive metabolites associated to bacterial growth inhibition.

Copyright

© 2011 Reynaldo De La Cruz-Quiroz, Catalina Chavez, Marcela Hernandez, Raul Rodriguez, Daniel Hernandez and Cristobal Noe Aguilar. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.