DNA Polymorphism of Bovine Pituitary-Specific Transcription Factor and Leptin Gene Between Iranian Bos indicus and Bos taurus Cattle
Arash Javanmard, Nader Asadzadeh and Fatollah Sarhadi
DOI : 10.3844/ajabssp.2010.282.285
American Journal of Agricultural and Biological Sciences
Volume 5, Issue 3
Problem statement: Variations at DNA level contribute to the genetic characterization of livestock populations and this may help to identify possible hybridization events as well as past evolutionary trends. Approach: The leptin and Pit-1 are attractive candidate genes for production and reproduction traits in cattle. A total of 247 Animals from four breeds from two species of Iranian cattle populations in include Bos taurus (Sarabi, Golpayegani) and Bos indicus (Sistani, Taleshi) were genotyped for the Pit-1 HinfI and leptin Sau3AI polymorphisms by the Polymerase Chain Reaction and Restriction Fragment Length Polymorphism (PCR-RFLP). Results: The genotype and gene frequencies for each group were determined and shown to be quite variable among the breeds. The allele B for the leptin gene and allele A for the Pit-1 gene were investigated high frequency in Bos indicus. Conclusion: Present result supported the previously proposed long evolutionary separation of these cattle subrace. Candidate gene approach may be a useful method to measure of genetic distance for cross breeding program between taurin and indicine cattle.
© 2010 Arash Javanmard, Nader Asadzadeh and Fatollah Sarhadi. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.