Comparative Permeation Studies between Scale Region of Shed Snake Skin and Human Skin In vitro
Aroonsri Priprem, Chidchanok Khamlert, Thaned Pongjanyakul, Sarayut Radapong, Theera Rittirod and Padungkwan Chitropas
DOI : 10.3844/ajabssp.2008.444.450
American Journal of Agricultural and Biological Sciences
Volume 3, Issue 1
Scales of shed king cobra (Ophiophagus hannah) skin from the dorsal portions, (SSS) and human breast epidermis (HE) were used as barrier membranes for comparison in an in vitro drug permeation study of nine active compounds (MW range 150-300 g mol-1, pKa 3-10). Each compound, saturated in a donor solution at pH 4.0 or 5.6, permeated through the barrier membrane, fully hydrated. A receptor solution at pH 7.4 was sampled for quantification at its λmax by UV-Visible spectrophotometry and/or HPLC. The permeability coefficients of nine compounds were correlated to the n-octanol/water partition coefficients of these compounds. The permeability coefficient of these compounds using HE and SSS was correlated. Scales of shed skin from the dorsal portions of king cobras were shown to be well correlated to the human breast epidermis in this in vitro aqueous permeation study of these compounds.
© 2008 Aroonsri Priprem, Chidchanok Khamlert, Thaned Pongjanyakul, Sarayut Radapong, Theera Rittirod and Padungkwan Chitropas. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.